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axio observer z1 inverted epifluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axio observer z1 inverted epifluorescence microscope
    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative <t>epifluorescence</t> images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).
    Axio Observer Z1 Inverted Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+z1+inverted+epifluorescence+microscope/bio_rxiv__64898__2026__01__26__701753-142-33-39?v=Carl+Zeiss
    Average 99 stars, based on 4719 article reviews
    axio observer z1 inverted epifluorescence microscope - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Extracellular vesicles from triple negative breast cancer cells disrupt the blood–brain barrier via miR-146a-5p- and TGF-β1-mediated downregulation of endothelial Paqr5"

    Article Title: Extracellular vesicles from triple negative breast cancer cells disrupt the blood–brain barrier via miR-146a-5p- and TGF-β1-mediated downregulation of endothelial Paqr5

    Journal: bioRxiv

    doi: 10.64898/2026.01.26.701753

    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative epifluorescence images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).
    Figure Legend Snippet: Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative epifluorescence images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).

    Techniques Used: Control, Cell Culture, Pore Size, Immunofluorescence



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    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative <t>epifluorescence</t> images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).
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    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative <t>epifluorescence</t> images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).
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    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative <t>epifluorescence</t> images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).
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    Image Search Results


    Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative epifluorescence images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).

    Journal: bioRxiv

    Article Title: Extracellular vesicles from triple negative breast cancer cells disrupt the blood–brain barrier via miR-146a-5p- and TGF-β1-mediated downregulation of endothelial Paqr5

    doi: 10.64898/2026.01.26.701753

    Figure Lengend Snippet: Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative epifluorescence images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).

    Article Snippet: Cells from the top side of the filters were removed with a cotton swab and breast cancer cells migrated through the endothelial monolayer and the pores of the filter were imaged with an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a STEDYCON laser scanning confocal microscope (Abberior Instruments, Göttingen, Germany).

    Techniques: Control, Cell Culture, Pore Size, Immunofluorescence